The star had come under fire for planning her performance in Poland on the day of the the Assumption of Mary feast - a special holy date in the Catholic Church - and representatives for the Catholic community urged the star to move her Sticky & Sweet show to another day.
She refused and was slammed by ex-president Lech Walesa and nationalist group Pro Polonia, who branded her a "crypto-Satanist".
A group of protesters held up banners outside the concert venue, but Madonna defiantly hit the stage on Saturday and told the adoring crowd, "I feel your love!"
Devoted fans at the concert sang "May You Live A Hundred Years," a traditional Polish birthday song, to mark the singer turning 51 on Sunday.
